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Promega pgl-3 promoter
Pgl 3 Promoter, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pgl-3+promoter/pgl3+basic/pmc11761945-94-22-24
Average 90 stars, based on 1 article reviews
pgl-3 promoter - by Bioz Stars, 2026-09
90/100 stars

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Related Articles

Plasmid Preparation:

Article Title: Exploring the Therapeutic Potential of Cannabidiol in U87MG Cells: Effects on Autophagy and NRF2 Pathway
Article Snippet: The pGL3-2xARE reporter plasmid was prepared by cloning a DNA fragment containing two binding motifs for the NRF2 transcription factor into the pGL-3 promoter (Promega, Madison, WI, USA) at the KpnI and BglII sites [ ].

Article Title: Scurfin (FOXP3) Acts as a Repressor of Transcription and Regulates T Cell Activation
Article Snippet: Restriction sites were included at the ends of the oligonucleotides to facilitate subcloning of the multimer into the SV40 promoter-driven luciferase reporter, pGL-3 Promoter (Promega, Madison, WI) to create 3xFKHluc.

Article Title: Exploring the Neuroprotective Potential of N-Methylpyridinium against LPS-Induced Neuroinflammation: Insights from Molecular Mechanisms
Article Snippet: The pGL3-2xNFκB reporter plasmid was obtained by cloning a DNA fragment containing 2 binding motifs for NF-κB transcription factor into the pGL-3 promoter (Promega, Madison, WI, USA) at the KpnI and BglII sites.

Article Title: pp. 88–154
Article Snippet: Mouse counterparts of the conserved genetic elements CR3, CR4, and CR5 were amplified by PCR from genomic mouse DNA and cloned into pGL-3 promoter (Promega).

Cloning:

Article Title: Exploring the Therapeutic Potential of Cannabidiol in U87MG Cells: Effects on Autophagy and NRF2 Pathway
Article Snippet: The pGL3-2xARE reporter plasmid was prepared by cloning a DNA fragment containing two binding motifs for the NRF2 transcription factor into the pGL-3 promoter (Promega, Madison, WI, USA) at the KpnI and BglII sites [ ].

Article Title: Scurfin (FOXP3) Acts as a Repressor of Transcription and Regulates T Cell Activation
Article Snippet: Restriction sites were included at the ends of the oligonucleotides to facilitate subcloning of the multimer into the SV40 promoter-driven luciferase reporter, pGL-3 Promoter (Promega, Madison, WI) to create 3xFKHluc.

Article Title: Exploring the Neuroprotective Potential of N-Methylpyridinium against LPS-Induced Neuroinflammation: Insights from Molecular Mechanisms
Article Snippet: The pGL3-2xNFκB reporter plasmid was obtained by cloning a DNA fragment containing 2 binding motifs for NF-κB transcription factor into the pGL-3 promoter (Promega, Madison, WI, USA) at the KpnI and BglII sites.

Article Title: pp. 88–154
Article Snippet: Mouse counterparts of the conserved genetic elements CR3, CR4, and CR5 were amplified by PCR from genomic mouse DNA and cloned into pGL-3 promoter (Promega).

Binding Assay:

Article Title: Exploring the Therapeutic Potential of Cannabidiol in U87MG Cells: Effects on Autophagy and NRF2 Pathway
Article Snippet: The pGL3-2xARE reporter plasmid was prepared by cloning a DNA fragment containing two binding motifs for the NRF2 transcription factor into the pGL-3 promoter (Promega, Madison, WI, USA) at the KpnI and BglII sites [ ].

Article Title: Scurfin (FOXP3) Acts as a Repressor of Transcription and Regulates T Cell Activation
Article Snippet: Restriction sites were included at the ends of the oligonucleotides to facilitate subcloning of the multimer into the SV40 promoter-driven luciferase reporter, pGL-3 Promoter (Promega, Madison, WI) to create 3xFKHluc.

Article Title: Exploring the Neuroprotective Potential of N-Methylpyridinium against LPS-Induced Neuroinflammation: Insights from Molecular Mechanisms
Article Snippet: The pGL3-2xNFκB reporter plasmid was obtained by cloning a DNA fragment containing 2 binding motifs for NF-κB transcription factor into the pGL-3 promoter (Promega, Madison, WI, USA) at the KpnI and BglII sites.

Article Title: pp. 88–154
Article Snippet: Mouse counterparts of the conserved genetic elements CR3, CR4, and CR5 were amplified by PCR from genomic mouse DNA and cloned into pGL-3 promoter (Promega).

Amplification:

Article Title: Exploring the Therapeutic Potential of Cannabidiol in U87MG Cells: Effects on Autophagy and NRF2 Pathway
Article Snippet: The pGL3-2xARE reporter plasmid was prepared by cloning a DNA fragment containing two binding motifs for the NRF2 transcription factor into the pGL-3 promoter (Promega, Madison, WI, USA) at the KpnI and BglII sites [ ].

Article Title: Scurfin (FOXP3) Acts as a Repressor of Transcription and Regulates T Cell Activation
Article Snippet: Restriction sites were included at the ends of the oligonucleotides to facilitate subcloning of the multimer into the SV40 promoter-driven luciferase reporter, pGL-3 Promoter (Promega, Madison, WI) to create 3xFKHluc.

Article Title: Exploring the Neuroprotective Potential of N-Methylpyridinium against LPS-Induced Neuroinflammation: Insights from Molecular Mechanisms
Article Snippet: The pGL3-2xNFκB reporter plasmid was obtained by cloning a DNA fragment containing 2 binding motifs for NF-κB transcription factor into the pGL-3 promoter (Promega, Madison, WI, USA) at the KpnI and BglII sites.

Article Title: pp. 88–154
Article Snippet: Mouse counterparts of the conserved genetic elements CR3, CR4, and CR5 were amplified by PCR from genomic mouse DNA and cloned into pGL-3 promoter (Promega).

Polymerase Chain Reaction:

Article Title: Exploring the Therapeutic Potential of Cannabidiol in U87MG Cells: Effects on Autophagy and NRF2 Pathway
Article Snippet: The pGL3-2xARE reporter plasmid was prepared by cloning a DNA fragment containing two binding motifs for the NRF2 transcription factor into the pGL-3 promoter (Promega, Madison, WI, USA) at the KpnI and BglII sites [ ].

Article Title: Scurfin (FOXP3) Acts as a Repressor of Transcription and Regulates T Cell Activation
Article Snippet: Restriction sites were included at the ends of the oligonucleotides to facilitate subcloning of the multimer into the SV40 promoter-driven luciferase reporter, pGL-3 Promoter (Promega, Madison, WI) to create 3xFKHluc.

Article Title: Exploring the Neuroprotective Potential of N-Methylpyridinium against LPS-Induced Neuroinflammation: Insights from Molecular Mechanisms
Article Snippet: The pGL3-2xNFκB reporter plasmid was obtained by cloning a DNA fragment containing 2 binding motifs for NF-κB transcription factor into the pGL-3 promoter (Promega, Madison, WI, USA) at the KpnI and BglII sites.

Article Title: pp. 88–154
Article Snippet: Mouse counterparts of the conserved genetic elements CR3, CR4, and CR5 were amplified by PCR from genomic mouse DNA and cloned into pGL-3 promoter (Promega).

Clone Assay:

Article Title: Exploring the Therapeutic Potential of Cannabidiol in U87MG Cells: Effects on Autophagy and NRF2 Pathway
Article Snippet: The pGL3-2xARE reporter plasmid was prepared by cloning a DNA fragment containing two binding motifs for the NRF2 transcription factor into the pGL-3 promoter (Promega, Madison, WI, USA) at the KpnI and BglII sites [ ].

Article Title: Scurfin (FOXP3) Acts as a Repressor of Transcription and Regulates T Cell Activation
Article Snippet: Restriction sites were included at the ends of the oligonucleotides to facilitate subcloning of the multimer into the SV40 promoter-driven luciferase reporter, pGL-3 Promoter (Promega, Madison, WI) to create 3xFKHluc.

Article Title: Exploring the Neuroprotective Potential of N-Methylpyridinium against LPS-Induced Neuroinflammation: Insights from Molecular Mechanisms
Article Snippet: The pGL3-2xNFκB reporter plasmid was obtained by cloning a DNA fragment containing 2 binding motifs for NF-κB transcription factor into the pGL-3 promoter (Promega, Madison, WI, USA) at the KpnI and BglII sites.

Article Title: pp. 88–154
Article Snippet: Mouse counterparts of the conserved genetic elements CR3, CR4, and CR5 were amplified by PCR from genomic mouse DNA and cloned into pGL-3 promoter (Promega).

Subcloning:

Article Title: Exploring the Therapeutic Potential of Cannabidiol in U87MG Cells: Effects on Autophagy and NRF2 Pathway
Article Snippet: The pGL3-2xARE reporter plasmid was prepared by cloning a DNA fragment containing two binding motifs for the NRF2 transcription factor into the pGL-3 promoter (Promega, Madison, WI, USA) at the KpnI and BglII sites [ ].

Article Title: Scurfin (FOXP3) Acts as a Repressor of Transcription and Regulates T Cell Activation
Article Snippet: Restriction sites were included at the ends of the oligonucleotides to facilitate subcloning of the multimer into the SV40 promoter-driven luciferase reporter, pGL-3 Promoter (Promega, Madison, WI) to create 3xFKHluc.

Article Title: Exploring the Neuroprotective Potential of N-Methylpyridinium against LPS-Induced Neuroinflammation: Insights from Molecular Mechanisms
Article Snippet: The pGL3-2xNFκB reporter plasmid was obtained by cloning a DNA fragment containing 2 binding motifs for NF-κB transcription factor into the pGL-3 promoter (Promega, Madison, WI, USA) at the KpnI and BglII sites.

Article Title: pp. 88–154
Article Snippet: Mouse counterparts of the conserved genetic elements CR3, CR4, and CR5 were amplified by PCR from genomic mouse DNA and cloned into pGL-3 promoter (Promega).

Luciferase:

Article Title: Exploring the Therapeutic Potential of Cannabidiol in U87MG Cells: Effects on Autophagy and NRF2 Pathway
Article Snippet: The pGL3-2xARE reporter plasmid was prepared by cloning a DNA fragment containing two binding motifs for the NRF2 transcription factor into the pGL-3 promoter (Promega, Madison, WI, USA) at the KpnI and BglII sites [ ].

Article Title: Scurfin (FOXP3) Acts as a Repressor of Transcription and Regulates T Cell Activation
Article Snippet: Restriction sites were included at the ends of the oligonucleotides to facilitate subcloning of the multimer into the SV40 promoter-driven luciferase reporter, pGL-3 Promoter (Promega, Madison, WI) to create 3xFKHluc.

Article Title: Exploring the Neuroprotective Potential of N-Methylpyridinium against LPS-Induced Neuroinflammation: Insights from Molecular Mechanisms
Article Snippet: The pGL3-2xNFκB reporter plasmid was obtained by cloning a DNA fragment containing 2 binding motifs for NF-κB transcription factor into the pGL-3 promoter (Promega, Madison, WI, USA) at the KpnI and BglII sites.

Article Title: pp. 88–154
Article Snippet: Mouse counterparts of the conserved genetic elements CR3, CR4, and CR5 were amplified by PCR from genomic mouse DNA and cloned into pGL-3 promoter (Promega).



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